2900 E. Broadway Blvd.
If you couldn't tell by the name, this place knows comedy. Tucson's oldest comedy club is also Arizona's longest continuously running laugh house, and draws nationally touring shows twice a week. If you're more interested in finding next year's big stars, head out for Thursday night open mic; you never know who may walk on the stage. From Ellen Degeneres to Tim Allen, they have all had their time on the Laff's stage.
Runners up:
2. Tucson Imrov Movement
3. Unscrewed Theater